c-di-GMP Signaling: Methods and Protocols 1st ed. 2017 Edition

Ebook Details

Authors

Karin Sauer

Year 2017
Pages 486
Publisher Humana Press
Language en
ISBN 9781493972395
File Size 8.25 MB
File Format PDF
Download Counter 36
Amazon Link

Ebook Description

This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling. The chapters, divided into eight major parts, guide readers through methods on synthesis, detection, quantitation, modulation of the levels of c-di-GMP present in cells, procedures to detect and evaluate the interaction of c-di-GMP, and up and coming approaches focusing on the inhibition of c-di-GMP signaling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.